|
|
|
| Registros recuperados: 65 | |
|
| |
|
|
| Carrillo-Nieto,Oscar; Domínguez-Vara,Ignacio A.; Huerta-Bravo,Maximino; Jaramillo-Escutia,Gerardo; Díaz-Zarco,Soledad; Vázquez-Armijo,José Fernando; Pescador-Salas,Nazario; Revilla-Vázquez,Alma. |
| Resumen En México se han diagnosticado deficiencias de minerales traza en ovinos en pastoreo, pero hay pocas estrategias para corregirlas y evaluar su respuesta. El objetivo de este estudio fue evaluar, en sementales ovinos (10 Hampshire con PV 80.6±7.6 kg y 8 Suffolk con PV 86.3±9.3 kg), el efecto de un suplemento de selenito de sodio en un bolo intraruminal de liberación prolongada (500 mg de Se), sobre el PV, condición corporal, circunferencia escrotal, variables hemáticas, actividad de glutatión peroxidasa, concentración de selenio en suero sanguíneo y características del eyaculado. El diseño experimental fue completamente al azar con arreglo factorial 2x2x3 sin y con Se (bolo intraruminal), razas (Hampshire y Suffolk) y tres meses en la época... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Carneros; Selenito de sodio; Bolo intraruminal; Semen; Glutatión peroxidasa. |
| Ano: 2018 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952018000600827 |
| |
|
|
| Lucio,Rosa Angélica; Tlachi,José Luis; López,Aurora Adriana; Zempoalteca,René; Velázquez-Moctezuma,Javier. |
| Se ha cuantificado en el eyaculado de la rata de laboratorio, el número de espermatozoides, peso y tamaño del tapón seminal, y en muestras obtenidas de epidídimo, la viabilidad, movilidad y concentración espermáticas. Los valores de referencia para el eyaculado de la rata no se han determinado quizá por la dificultad para obtenerlo directamente del macho. No obstante, puede obtenerse una aproximación al analizar el eyaculado recolectado de una hembra recién inseminada. El objetivo de este estudio fue evaluar y proponer los valores de los parámetros macroscópicos y microscópicos del semen y tapón seminal obtenidos de la hembra. El análisis del semen de ratas Wistar se realizó adecuando los métodos usados para otras especies. Después de una serie... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Semen; Movilidad espermática; Viabilidad espermática; Morfología espermática; Número de espermatozoides; Tapón seminal. |
| Ano: 2009 |
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S0301-50922009000400007 |
| |
|
|
| Uberti,Marcela Fornari; Vieira,Felipe do Nascimento; Salência,Helena Ragibo; Vieira,Genyess da Silva; Vinatea,Luis Alejandro. |
| Aiming at assessing the cryopreservation potential of Litopenaeus vannamei sperm cells, 74 spermatophores were manually extracted from the sexually mature individuals. After the toxicity test to define the cryoprotectant concentration, suspensions of spermatic cells were cryopreserved in the groups in freezing solutions comprising different cryoprotectants such as dimethyl sulfoxide (DMSO) and ethylene glycol (EG) at 10% concentration. Each treatment was divided in subgroups for storage in liquid nitrogen during 0, 30, 60 and 90 days, in triplicate. After thawing at 25ºC for 40 seconds, cell viability in the suspensions was analyzed under the microscope in eosin-nigrosin stain and flow cytometry. There were no significant differences between the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cryogenics; Semen; Marine shrimp; Cell viability. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300010 |
| |
|
| |
|
|
| Henzel,A.; Salla,P.F.; Mascitti,A.K.; Demoliner,M.; Solyman,M.C.; Lunge,V.R.; Spilki,F.R.. |
| ABSTRACT Bovine alphaherpesviruses 1 and 5 (BoHV-1/5) are main pathogens of respiratory, reproductive and neurological diseases in cattle. The aim of this study was to investigate the frequency of neutralizing antibodies against BoHV-1/5 in serum samples and to detect viral DNA in semen of bulls from beef cattle farms located in RS. A total of 372 serum and semen sample from bulls were collected in eighteen farms. Serum samples were submitted to virus neutralization (VN) assay, while semen samples were used to detect BoHV-1 and BoHV-5 DNA by PCR. VN results showed that BoHV-1/5 antibodies were detected in bulls of 66.7% (12/18) of the farms, 295 (79.5%) BoHV positive bulls, 287 for BoHV-1 and 234 for BoHV-; at 43 vaccinated bulls 72.1% (31/43) showing... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: BoHV-1 and 5; Co-infection; DNA; Semen. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000100197 |
| |
|
|
| Pereira, Marcelo Maia; Universidade Federal de Viçosa; Ribeiro Filho, Oswaldo Pinto; Universidade Federal de Viçosa; Zanuncio, Jose Cola; Universidade Federal de Viçosa; Troni, Allan Reis; Universidade Federal de Viçosa; Navarro, Rodrigo Diana; Universidade de Brasília; Takamura, Angela Emi; Universidade Federal de Viçosa. |
| By understanding the hormonal action behind the induction of spermiation in Leptodactylus ocellatus can help in the collection of semen for artificial insemination and cryopreservation. The experiments were conducted at the Experimental Frog Farm of the Federal University of Viçosa. Seven L. ocellatus males with the following secondary sexual characteristics were selected: black thorns, developed forelimbs, and amplexus reflex. Spermiation was induced in these animals by applying daily 0.4 μg buserelin acetate hormone (GnRHa) for 7 days, being the semen collected 90 min after the application. The volume, color, vigor, motility, sperm concentration, and spermatozoa morphology were subsequently evaluated. Three animals responded to GnRHa induction, enabling... |
| Tipo: Estudos morfológicos |
Palavras-chave: 2.04.06.00-2; 5.05.04.00-2; 5.06.03.00-0 creole frog; GnRHa; Semen; Spermatozoon; Sperm motility Aquicultura. |
| Ano: 2012 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14777 |
| |
|
|
| Villatoro Salinas, Roberto de Jesús. |
| Las características seminales de sementales Criollo Lechero Tropical (CLT) son poco conocidas y son requeridas para producir dosis de semen para inseminación artificial (IA) y sementales de alto valor genético todo el año. El objetivo del presente estudio fue caracterizar el semen de sementales CLT en dos épocas del año, fresca (enero) y calurosa (mayo) en la región central de Veracruz, México, de clima cálido subhúmedo, precipitación y temperatura media anual de 1060 mm y 26.4 oC. Se utilizaron 70 muestras de semen provenientes de siete sementales de 25.6±1.68 meses de edad y 319.14±6.24 kg, alimentados en pastoreo de zacate Pará (Brachiaria mutica) y un suplemento comercial con 18 % de proteína (4 kg d-1 animal-1). Se recolectaron cinco muestras de semen... |
|
Palavras-chave: Semen; Bovino criollo; Climas cálidos; Criollo cattle; Warm climates; Veracruz; Ganadería; Maestría. |
| Ano: 2013 |
URL: http://hdl.handle.net/10521/1849 |
| |
|
|
| Silva,E.C.B.; Arruda,L.C.P.; Vieira,J.I.T.; Soares,P.C.; Guerra,M.M.P.. |
| ABSTRACT The aim of this study was to evaluate the effects of different concentrations of (+)-catechin or (-)-epigallocatechin gallate (EGCG) on goat semen freezability. Poolsof semen were processed (Experiment 1: 0, 15, 25, 50, 75, or 100µM (+)-catechin; Experiment 2: 0, 15, 25, 50, 75, or 100µM EGCG) and frozen. After thawing, the samples were evaluated for kinematics, plasma membrane (PMi) and acrosome integrity, morphology, and oxidative stress, at 0 and 1h. In Experiment 1, at 0h, VSL and VAP were greater (P<0.05) with 15µM than with 50 and 100; WOB was lower (P<0.05) with 100µM than with 0, 15, and 25; and BCF was higher (P<0.05) with 75 and 100µM than with 0. In turn, in Experiment 2, progressive motility was higher (P<0.05) with0 and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antioxidants; Cryopreservation; Flavonoids; Oxidative stress; Semen. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000200521 |
| |
|
|
| Silva,A.R.; Cardoso,R.C.S.; Silva,L.D.M.. |
| Standardized sperm concentration and volume:volume extension were compared as dilution rates for canine semen freezing. Six proven stud dogs were submitted to two seminal collections by manual stimulation. Semen was evaluated and extended in tris plus egg-yolk and glycerol according to two different dilution rates. The first one was based on a standardized sperm concentration of 200x10(6) spermatozoa/ml and the second was a volume:volume extension at a proportion of one part semen to one part extender. Semen was frozen, stored in liquid nitrogen and thawed after one week. Sperm motility and vigor were appraised after each stage of the process and at 15 and 30min post-thawing. Sperm morphology was analyzed after collection and thawing. No differences were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Dog; Semen; Extender; Freezing; Dilution. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352005000600010 |
| |
|
|
| SANTOS,Silvia Edelweiss Crusco dos; VANNUCCHI,Camila Infantosi; SATZINGER,Sandra; ASSUMPÇÃO,Mayra Elena Ortiz DAvila; VISINTIN,José Antonio. |
| Semen was collected from six stud dogs to compare five extenders in the semen freezing process. Each ejaculate was divided in five parts and added to tris-fructose-citric acid, glicine, lactose, skim milk and tris-fructose-citrate extenders. The semen was diluted at 37°C in extenders without glycerol, in the ratio 1:1 and cooled for 60 minutes to reach a 5°C temperature. Then, extenders with glycerol in the ratio of 2:1 were added to give the final prefreezing concentration of 4% of glycerol. The diluted semen with the cryoprotectant was maintained for a further 60 minutes in refrigeration to equilibrate the spermatozoa in the glycerol and packaged in 0.5 ml plastic straws. The straws were maintained for 30 minutes in vapor, plunged and stored in liquid... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diluents; Freezing; Semen; Dogs. |
| Ano: 1999 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95961999000500004 |
| |
|
|
| Miraglia,Fabiana; Morais,Zenaide Maria; Cortez,Adriana; Melville,Priscilla Anne; Marvullo,Maria Fernanda Vianna; Richtzenhain,Leonardo José; Visintin,José Antonio; Vasconcellos,Silvio Arruda. |
| Inactivation of leptospires in pools of semen from three Holstein Friesian bulls, collected in an artificial vagina, was investigated. Spermatic concentration was adjusted in egg yolk citrate extender, submitted to the following treatments: A (control; without antibiotics); B (penicillin, 1,000 UI/mL - streptomycin, 1,000 µg/mL); C (amoxicillin, 1,000 µg/mL); D (ceptiofur sodium, 1,000 µg/mL); E (amoxicillin 1,000 µg/mL - ceptiofur sodium 1,000 µg/mL). Leptospires (2.0 x 10(6) leptospires/mL) were added into the diluted semen. Recovery of leptospires was obtained in modified EMJH semi-solid medium with and without antibiotics. The antibiotics in the concentrations used did not affect means of percentage of progressive motility and individual progressive... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospires; Semen; Antibiotics; Extender. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000200011 |
| |
|
|
| Hogeland, Julie A.. |
| The artificial insemination industry provides breeding products and services for both dairy and beef cattle. Two-thirds of the industry is organized as producer-owned cooperatives. The industry has been so highly successful in meeting the needs of producers for a high-quality reliable product that the United States is the world's leading producer and exporter of bull semen. Yet, more bulls (and consequently more cooperatives) are available than are technically needed for genetic variation and breeding requirements. The result of such product proliferation is higher semen prices and excessive costs for inventory, distribution, and marketing. To maintain their prominent role in the industry, the 22 cooperatives need to consolidate to streamline the... |
| Tipo: Report |
Palavras-chave: Cooperatives; Artificial insemination; Semen; Dairy cattle; Breeding; Agribusiness. |
| Ano: 1990 |
URL: http://purl.umn.edu/52019 |
| |
|
| |
|
|
| Ninhaus-Silveira,Alexandre; Foresti,Fausto; Tabata,Yara Aiko; Rigolino,Marcos Guilherme; Veríssimo-Silveira,Rosicleire. |
| Cryopreservation of semen from sex-reversed females of rainbow trout aims at rationalizing the production of stocks composed by 100% females. Semen from normal males (M) and two types of genotypic females (R and G), sex-reversed by the oral administration of 17alpha-methyltestosterone, were used. R was obtained by the fertilization of normal eggs with semen of sex-reversed females while G via gynogenetic reproduction. Semen was diluted in an extender solution (glucose 5,4 g, egg yolk 10 ml, dimetil sulfoxide 10 ml, water 80 ml) at 1:3 ratio (semen/extender), stored in straws of 0.5 ml and freezed in a dry container Cryopac CP-65, at -180ºC. Thawing was performed with water at 70ºC for 3 seconds. There were no significant fertilization rate differences... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oncorhynchus mykiss; Cryopreservation; Sex reversal; Gynogenesis; Semen. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100009 |
| |
|
|
| Neves,M.M.; Heneine,L.G.D.; Henry,M.. |
| The aim of this study was to evaluate the use of low concentrations of natural and lyophilized low density lipoprotein (LDL) from hen's egg yolk for cryopreservation of canine semen. Different ammonium sulphate concentrations were tested to extract LDL from egg yolk. The yolk was centrifuged, and LDL was isolated using 10, 20, 40, 45, or 50% ammonium sulphate solution (ASS). The LDL-rich floating fraction was collected for chemical characterization. Dry matter content was lowest (P<0.05) in the LDL extracted with the 50% ASS. The purification of LDL increased in association with increasing ammonium sulphate concentrations. SDS-PAGE showed that the 50% ASS solution yielded a purer fraction of LDL from egg yolk. For semen cryopreservation, TRIS extender... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Egg Yolk; LDL extraction; Ammonium sulphate; Dog; Semen. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300769 |
| |
|
| |
|
| |
|
|
| Silva,L.C.; Assis,V.P.; Ribeiro,V.M.; Tafuri,W.L.; Toledo Júnior,J.C.; Silva,S.O.; Melo,M.N.; Rachid,M.A.; Valle,G.R.. |
| Considering the venereal transmission of visceral leishmaniasis from dogs to bitches, the aim of this study was to verify if the penile surface and smegma from infected dogs can be the source of parasites in bitches. Twelve Leishmania infantum infected dogs had semen and smegma samples collected for submission to PCR identification of the DNA of the parasite. Semen (41.7%) and smegma (50.0%) have similar positive incidence (P>0.05; Fisher's exact test), with 58.3% of the dogs positive for semen and/or smegma samples. The proportion of positivity for both semen and smegma was 33.3%, but 8.3% was positive only for semen, and 16.7% only for smegma, revealing a moderate agreement between tests (K=0.5; Kappa index). It was concluded that Leishmania infantum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leishmania infantum; Dog; Prepuce; Smegma; Semen. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352014000300731 |
| |
|
| |
| Registros recuperados: 65 | |
|
|
|
